the effects of in ovo feeding of amino acids, carbohydrate and butyric acid on intestinal morphology and growth performance of broiler chicks

in this research 400 ross 308 breeder fertile eggs were weighed at day 16 of incubation and evenly distributed into 4 treatments with 4 replicates per treatment and 25 eggs per replicate in a crd design. on day 18 of incubation, 1 ml of an in ovo feed solution including: i) carbohydrate (cho), ii) amino acid (aa), and iii) butyric acid solution in 0.5% saline was injected into amniotic fluid of eggs. the control group remained non-injected. after injection eggs were transferred to hatcher. upon hatch, chicks were weighed, sexed and transferred to the experimental house and reared to 42 days. at day two the midpoint of the jejunum was sampled for intestinal morphometric examination. the hatchability didn't differ significantly between treatments. body weight and body weight to egg weight ratio of all in ovo injected hatchlings was greater than in controls (p